DNA sequencing

Describe the limitations of traditional methods of Identifying bacteria. Traditional methods include judging by phenotypes characteristics. This could be a problem if two bacteria looked similar because they could easily be confused. Summarize the goal of each of the six parts of the lab. Sample Prep First we need to extract the DNA from the bacterial wall PC Amplification Create millions of copies of the initial DNA PC purification Filter out DNA from primers and enzymes Sequencing Prep Run more PC to make copies of DNA at various lengths DNA Sequencing

Sequence machine runs gel electrophoresis to Identify nucleotides and determine sequence. Sequence Analysis Computer determines full DNA sequence which can be run and compared. Explain how fluorescent markers help determine a nucleotide sequence. Each nucleotide is assigned a certain color. Conclusion 1 How can scientists identify specific bacteria when they are amplifying and studying the same region of DNA In each species? They can identify it because there are unique sequences for each species. If you take a sample and find the DNA of it you can then compare it to the Dona’s of known bacteria to discover what bacteria the sample is from